Queensland fruit fly (Q-fly) was detected in Loxton following the discovery of seven male flies.
A 1.5-kilometre outbreak area and 15-kilometre suspension area have been established around the detection point and quarantine restrictions apply in the Newton Road area.
Minister for Primary Industries and Regional Development Tim Whetstone said Primary Industries and Regions (PIRSA) staff had immediately commenced an eradication program aimed at eliminating fruit flies from the outbreak area and nearby surrounds.
"The State Government, industry and community members have run simulated outbreak exercises this year, which has assisted in preparing local people for the roles they need to play in swiftly eradicating this pest from the region," Mr Whetstone said.
"Residents and businesses within the outbreak area will be receiving information from PIRSA about the outbreak and associated quarantine restrictions, detailing what part they can play in preventing its spread.
"We are liaising closely with the horticulture industry in the Riverland, particularly in regards to the movement of produce and quarantine restrictions."
More to come.